Transcriptome analyses of Anguillicola crassus from native and novel hosts
نویسندگان
چکیده
Anguillicola crassus is a swim bladder nematode of eels. The parasite is native to the Asian eel Anguilla japonica, but was introduced to Europe and the European eel Anguilla anguilla in the early 1980s. A Taiwanese source has been proposed for this introduction. In the new host in the recipient area, the parasite appears to be more pathogenic. As a reason for these differences, genetically fixed differences in infectivity and development between Taiwanese and European A.crassus have been described and disentangled from plasticity induced by different host environments. To explore whether transcriptional regulation is involved in these lifecycle differences, we have analysed a "common garden", cross infection experiment, using deep-sequencing transcriptomics. Surprisingly, in the face of clear phenotypic differences in life history traits, we identified no significant differences in gene expression between parasite populations or between experimental host species. From 120,000 SNPs identified in the transcriptome data we found that European A. crassus were not a genetic subset of the Taiwanese nematodes sampled. The loci that have the major contribution to the European-Taiwanese population differentiation show an enrichment of synonymous and non-coding polymorphism. This argues against positive selection in population differentiation. However, genes involved in protein processing in the endoplasmatic reticulum membrane and genes bearing secretion signal sequences were enriched in the set of genes most differentiated between European and Taiwanese A. crassus. These genes could be a source for the phenotypically visible genetically fixed differences between European and Taiwanese A. crassus.
منابع مشابه
Competing invaders: Performance of two Anguillicola species in Lake Bracciano
Anguillicola crassus is one of the most successful parasitic invasive species as it has spread from its original habitat in East Asia throughout the world and has acquired five new eel host species in the course of its invasion within the last three decades. Records from an Italian lake indicate that this species has even displaced an established population of its close relative A. novaezelandi...
متن کاملEffect of salinity on hatching, survival and infectivity of Anguillicola crassus (Nematoda: Dracunculoidea) larvae.
The effect of salinity on hatching, larval survival and infectivity of Anguillicola crassus was studied under experimental conditions using eggs obtained from naturally infected eels. Egg hatching rate, second-stage larval survival and larval infectivity were maximal in fresh water and declined with increase in salinity. Larvae survived up to 100 d in fresh water, 70 d in 50 % sea water and 40 ...
متن کاملAmphibians and larvae of aquatic insects as new paratenic hosts of Anguillicola crassus (Nematoda: Dracunculoidea), a swimbladder parasite of eels.
Amphibians (tadpoles of the frog Bombina bombina [L.] and the newt Triturus vulgaris [L.]) and aquatic insects (larvae of the alderfly Sialis lutaria [L.] [Megaloptera], dragonflies Sympetrum sanguineum [Miller] and Coenagrion puella [L.] [Odonata], and the caddisfly Oligotrichia striati [L.] [Trichoptera]) were found to serve as paratenic hosts for the third-stage larvae (L3) of the nematode A...
متن کاملRelationship between European eel Anguilla anguilla infection with non-native parasites and swimming behaviour on encountering accelerating flow.
The effect of Anguillicola crassus, Pseudodactylogyrus bini and Pseudodactylogyrus anguillae infection on the behaviour of downstream migrating adult European eels Anguilla anguilla as they encountered accelerating water velocity, common at engineered structures where flow is constricted (e.g. weirs and bypass systems), was evaluated in an experimental flume. The probability of reacting to, and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 2 شماره
صفحات -
تاریخ انتشار 2014